Evaluation of silk fibroin/curcumin composite film for promoting wound healing / 中国组织工程研究

BACKGROUND: In the previous study, we have successfully prepared a silk fibroin/curcumin composite film, which possesses good physicochemical properties, sustained-release properties, antibacterial activity and biocompatibility. OBJECTIVE: To further observe the effect of the prepared silk fibroin/curcumin composite film, as the wound dressing, in the field of wound healing. METHODS: The silk fibroin/curcumin composite film was prepared by referring to the previously established methods. The mechanical properties and light transmittance of the prepared composite film were then investigated. Escherichia coli and Staphylococcus aureus were used as strains. The plate-counting technique was applied to evaluate the antibacterial activity of the light illuminated silk fibroin/curcumin composite film, silk fibroin/curcumin composite film without light exposure, and silk fibroin film without light exposure. The full-thickness excisional lesions were created on the dorsum of 20 Kunming mice. The wounds of 10 mice were covered with silk fibroin/curcumin composite film, and the wounds of other 10 mice were dressed with sterile gauze. The healing status and histological morphology of the wound sites were observed at 3, 7, and 14 days post operation. The animal experimental design and the protocols were approved by the Laboratory Animal Ethics Committee of Southwest University (approval number: IACUC-2019011). RESULTS AND CONCLUSION: (1) Compared to silk fibroin film, the fracture strength of silk fibroin/curcumin composite film increased significantly (P < 0.05), while the Young’s modulus of silk fibroin/curcumin composite film decreased significantly (P < 0.01). (2) Both silk fibroin film and the silk fibroin/curcumin composite film exhibited excellent transparency, but the silk fibroin/curcumin composite film showed a stronger absorption in visible light region at a wavelength of 400-450 nm. (3) Without light illumination, the silk fibroin/curcumin composite film exhibited stronger antibacterial activity than that of silk fibroin film. While, the silk fibroin/curcumin composite film with light illumination demonstrated stronger antibacterial activity than that of silk fibroin/curcumin composite film without light illumination. (4) The wound healing rate of the experimental group was higher than that of the control group at each time point (P <0.05). Analysis on histological morphology reveals that the length of regenerated epithelia of dermal tissue and the thickness of granulation tissue in the experimental group were greater than those in the control group at 3 and 7 days post operation (P < 0.01). While, the thickness of granulation tissue was smaller than that in the control group at 14 days post operation (P < 0.01). (5) The results indicated that the silk fibroin/curcumin composite film can facilitate skin wound healing.

Short-term curative effect of vacuum sealing drainage (VSD) combined with platelet rich plasma (PRP) for the treatment of the refractory wounds / 中国骨伤

<p><b>OBJECTIVE</b>To observe the efficacy of the treatment of refractory wound by VSD combined with platelet-rich plasma.</p><p><b>METHODS</b>From April 2010 to June 2012,15 patients with refractory wound were treated including 11 males and 4 females with an average age of 35.2 years old ranging from 18 to 45 years old. The formation time of wound was from 6 to 24 months, which was unhealed after long-term medication or repeated treatment. The VSD combined with autologous platelet-rich plasma was applied to treat the wound. The wound healing was an indicator and treatment and clinical features were summarized.</p><p><b>RESULTS</b>All the patients were followed up for 3 to 8 months (means 5 months). The wound of all patients were healed without recurrence.</p><p><b>CONCLUSION</b>The curative effect of VSD combined with platelet rich plasma for treatment of refractory wounds is obvious. It could reduce the treatment course and the treatment cost.</p>

Dietary calcium decreases plasma cholesterol level only in female but not in male hamster fed a high cholesterol diet / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate the effect of dietary calcium on plasma lipoprotein profile in castrated and ovariectomized hamsters.</p><p><b>METHODS</b>Male, castrated, female and ovariectomized hamsters (n=36 each group) were randomly divided into three sub-groups (n=12) and fed one of the three diets containing 0, 2, and 8 g calcium per kg diet for a period of six weeks. Changes in plasma lipoprotein profile were monitored at the end of week 0, 3 and 6.</p><p><b>RESULTS</b>Plasma total cholesterol (TC), non-high density lipoprotein cholesterol (non-HDL-C), triacylglycerols (TG) and TC/HDL-C were decreased only in intact female and ovariectomized hamsters. In contrast, three levels of dietary calcium had no effect on lipoprotein profiles in both intact male and castrated hamsters.</p><p><b>CONCLUSION</b>Beneficial modification of lipoprotein profile by dietary calcium was gender-dependent at least in hamsters.</p>

Application principles of hatchet skin flap for repairing tissue defect of cheek / 中华外科杂志

<p><b>OBJECTIVE</b>To evaluate the design principles, clinical results and significance of hatchet skin flaps for repairing tissue defects in different parts of cheek.</p><p><b>METHODS</b>The area of cheek was divided into three parts, P(I), P(II) and P(III), with vertical lines through the medial canthus and lateral canthus. Different kinds of hatchet skin flaps were designed to repair tissue defects in different part of cheek. The hatchet skin flaps were performed in 29 cases with tissue defects in different part of cheek from August 2005 to August 2009. There were 17 male and 12 female, aged from 19 to 81 years, with a mean age of (45 ± 16) years. The size of tissue defect ranged from 1.5 cm × 1.5 cm to 2.5 cm × 3.5 cm. Patients' satisfactions were evaluated with a questionnaire in 5 aspects:color and texture match, scar, morbidity, and function after 6 months operatively.</p><p><b>RESULTS</b>All the flaps survived completely with good color and tissue match. The facial contour was not altered obviously. Six to eighteen months later, all scars were almost invisible. All 29 patients were satisfied with their results.</p><p><b>CONCLUSIONS</b>The hatchet skin flap is one of the versatile reconstructive methods for repairing of medium and small defects in the three parts of cheek. Defects in different part of cheek should be repaired individually with hatchet flap based on characters of natural lines.</p>

Effect of the tumescent infiltration solution temperature on body temperature / 中华整形外科杂志

<p><b>OBJECTIVE</b>To evaluate the effect of tumescent infiltration solution temperature on core body temperature after liposuction.</p><p><b>METHODS</b>15 healthy female subjects were randomly divided into 2 groups to receive tumescent infiltration solution at 25 degrees C as group A, or at 37 degrees C as group B. All subjects were under epidural anesthesia. Vital signs, including core temperature, heart rate, respiratory rate and blood pressure, were monitored immediately, 1 hour, 2 hours, 3 hours, 4 hours and 8 hours after operation.</p><p><b>RESULTS</b>The core body temperature immediately, 1 hour, 2 hours and 3 hours after operation were (35.8 +/- 0.5) degrees C, (35.8 +/- 0.5) degrees C, (36.0 +/- 0.5) degrees C, (36.1 +/- 0.5) degrees C in group A, and (36.5 +/- 0.4) degrees C, (36.5 +/- 0.3) degrees C, (36.5 +/- 0.3) degrees C, (36.6 +/- 0.4) degrees C in group B, showing a significant difference between the two groups (P = 0.008, P = 0.008, P = 0.03, P = 0.033, respectively). There was no difference in body temperature 4 hours and 8 hours after operation and in heart rate, respiratory rate and blood pressure between the two groups (P > 0. 05).</p><p><b>CONCLUSIONS</b>The tumescent infiltration solutions at room temperature (25 degrees C) can decrease the core body temperature and increase surgical risk. It might not be good for rehabilitation. It is recommended to use tumescent infiltration solution at body temperature (37 degrees C) in liposuction.</p>

Red yeast rice increases excretion of bile acids in hamsters / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate the hypocholesterolemic activity of red yeast rice (RYR) and its underlying mechanism.</p><p><b>METHODS</b>Three groups of hamsters were fed either the control diet or one of the two experimental diets containing by weight 0.1% RYR (0.1RYR) or 0.3% RYR (0.3RYR). Blood (0.5 mL) was collected from the retro-orbital sinus into a heparinized capillary tube at the end of week 0, 3, and 6. Plasma lipoproteins were measured using enzymatic kits, while fecal neutral and acidic sterols were quantified using a gas-liquid chromatography.</p><p><b>RESULTS</b>Plasma total cholesterol was reduced by 12% in 0.1RYR group and by 18% in 0.3RYR group compared with the control value. Similarly, plasma triacylglycerol was decreased by 11% in 0.1RYR group and by 24% in 0.3RYR group. Western blotting analysis demonstrated that RYR had no effect on sterol regulatory element binding protein 2, liver X receptor, 3-hydroxy-3-methylglutary-CoA reductase, LDL receptor, and cholesterol-7alpha-hydroxylase. HPLC analysis confirmed that RYR contained 0.88% monacolin K. It was recently found that RYR supplementation increased excretion of fecal acidic sterols by 3-4 folds compared with the control value.</p><p><b>CONCLUSION</b>Hypocholesterolemic activity of RYR is mediated at least partially by enhancement of acidic sterol excretion.</p>

Application of hatchet flap for buccal tissue defect / 中华整形外科杂志

<p><b>OBJECTIVE</b>To evaluate the therapeutic effect of hatchet flap for buccal tissue defect.</p><p><b>METHODS</b>The hatchet flap was designed beside the tissue defect and advanced to cover the defect.</p><p><b>RESULTS</b>Since 2006, 13 cases were treated with primary healing and no flap loss. The size of the flaps ranged from 1.8 cm x 2.0 cm to 2.5 cm x 3.5 cm. All the cases were followed up for 3 months to 1.5 years. The postoperative appearance was satisfactory with inconspicuous scar.</p><p><b>CONCLUSIONS</b>Hatchet flap is very suitable for the buccal tissue defect with satisfactory cosmetic result. The facial natural figure is protected very well.</p>

Developmental and reproductive toxicity of soybean isoflavones to immature SD rats / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate the dose-dependent toxic effect of soybean isoflavone extracts (SIE) on reproductive development in immature rats.</p><p><b>METHODS</b>Growing male and female rats (n = 50 each, 4 weeks) were divided into five groups fed with a standard cereal-based diet and gastrogavaged daily with 0, 30, 150, 300, and 600 mg SIE/kg body weight, respectively, for 12 weeks. Body weight, organ weights, and serum level of estrogen and testosterone were measured.</p><p><b>RESULTS</b>Oral administration of SIE had no effect on food intake but decreased food efficiency ratio (P < 0.01). Suppression on body weight gain by SIE was dose-dependent and the effect was greater on male than on female rats (P < 0.01). SIE at high doses exhibited hepatotoxicity by increasing a relative liver weight, and also caused a smaller uterus but a greater relative ovary in female rats, while leading to larger relative testis and epididymis in male rats. SIE could decrease progesterone concentrations in female rats, whereas in male rats it reduced not only total testosterone level but also sperm count compared with the control group (P < 0.05).</p><p><b>CONCLUSION</b>SIE at a range of 50-1000 times of human intake level affects not only growth but also development of reproductive system in growing rats.</p>

DHA depletion in rat brain is associated with impairment on spatial learning and memory / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To examine the effect of docosahexaenoic acid (DHA) deficiency in brain on spatial learning and memory in rats.</p><p><b>METHODS</b>Sprague Dawley rats were fed with an n-3 fatty acid deficient diet for two generations to induce DHA depletion in brain. DHA in seven brain regions was analyzed using the gas-liquid chromatography. Morris water maze (MWM) was employed as an assessing index of spatial learning and memory in the n-3 fatty acid deficient adult rats of second generation.</p><p><b>RESULTS</b>Feeding an n-3 deficient diet for two generations depleted DHA differently by 39%-63% in the seven brain regions including cerebellum, medulla, hypothalamus, striatum, hippocampus, cortex and midbrain. The MWM test showed that the n-3 deficient rats took a longer time and swam a longer distance to find the escape platform than the n-3 Adq group.</p><p><b>CONCLUSION</b>The spatial learning and memory in adult rats are partially impaired by brain DHA depletion.</p>

Isolation and elucidation of antioxidant constituents from acetone extract in root of Scutellaria rehderiana / 中国中药杂志

<p><b>OBJECTIVE</b>To isolatate and elucidate the antioxidant constituents from acetone extract of Scutellaria rehderiana.</p><p><b>METHOD</b>Chemical constituents were isolated by several column chromatographies and their structures were elucidated on the basis of standard compounds and spectral analysis.</p><p><b>RESULT</b>Five compounds, oroxylin A, wogonin, baicalein, ganhuangenin, ganhuangemin were isolated from acetone extract of S. rehderiana. Baicalin was obtained from methanol extract of S. rehderiana.</p><p><b>CONCLUSION</b>Ganhuangemin was isolated from the plant for the first time. Baicalein and ganhuangenin have stronger antioxidant activity than that of BHT (butylated hydroxytoluene).</p>

Change of immunological response and cell proliferation in breast augmented by polyacrylamide hydrogel injection / 第二军医大学学报

Objective:To study the change of immunological response and cell proliferation in breast tissues augmented by polyacrylamide hydrogel injection(PHI).Methods:The expression of CD68,CD25 and PCNA in 20 breast tissues with indurations,12 without indurations after breast augmentation by PHI,and 10 normal breast tissues was examined by immunohistochemistry P-V6000; analysis was also done by H-E staining.Results:Hyperplasia of fibrous tissue and infiltration of inflammatory cells and macrophages were found in the breast and adjacent tissues 3-8 years after PHI.Positive cells of CD68,CD25 and PCNA hardly existed in the normal tissues,but the breast tissues around the polyacrylamide hydrogel had many positive cells of CD68 and PCNA,especially in cases with indurations;there were significant differences between the 3 groups(P